Human FOLR2 ELISA Kit (ab267621)
Key features and details
- Sensitivity: 0.17 ng/ml
- Range: 0.156 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FOLR2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.17 ng/ml -
Range
0.156 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103.7 81% - 120% Serum 110.8 84% - 134% Plasma 74.2 71% - 76% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human FOLR2 ELISA Kit is designed for the quantitative determination of FOLR2 in cell culture supernatants, plasma and serum samples. This assay employs an antibody specific for human FOLR2 coated on a 96-well plate. Standards and samples are pipetted into the wells and FOLR2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FOLR2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FOLR2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Δ Note: Collect plasma using citrate and heparin as an anticoagulant. EDTA is not recommended as anticoagulants for use in this assay.
This ELISA kit shows no cross-reactivity with any of the cytokines tested: human ALK-1, B7- H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Human FOLR2 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human FOLR2 Detection Antibody 2 vials Human FOLR2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. -
Tissue specificity
Expressed in placenta and hematopoietic cells. Expression is increased in malignant tissues. -
Sequence similarities
Belongs to the folate receptor family. -
Post-translational
modificationsEight disulfide bonds are present.
N-glycosylated. -
Cellular localization
Cell membrane. Secreted. - Information by UniProt
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Alternative names
- beta hFR
- FBP
- FBP/PL 1
see all -
Database links
- Entrez Gene: 2350 Human
- Omim: 136425 Human
- SwissProt: P14207 Human
- Unigene: 433159 Human
Images
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Example standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.