Human Follistatin ELISA Kit (ab192147)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 28 pg/ml
- Range: 0.08 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Follistatin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 6.3% Inter-assay Sample n Mean SD CV% Overall 3 2.5% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
28 pg/ml -
Range
0.08 ng/ml - 5 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 110 99% - 125% Cell culture media 86 85% - 86% EDTA Plasma 92 89% - 95% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Follistatin ELISA Kit (ab192147) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Follistatin protein in cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Follistatin with 28 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Follistatin is a single-chain polypeptide consisting of about 344 amino acids. The follistatin protein is encoded by a relatively small gene size of 6 kb and 6 exons with alternative splice variants and post translational modifications to give a variety of molecular weights between 31 – 39 kDa. Follistatin forms a high affinity complex with activin sequestering it from its receptor thus having an inhibitory effect. Follistatin acts to promote muscle growth in mammals by acting as an antagonist to the protein myostatin. Myostatin acts as an inhibitor to muscle growth.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Follistatin Capture Antibody 1 x 600µl 10X Human Follistatin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Follistatin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Activin binding protein
- Follistatin isoform FST317
- FS
- FST
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Database links
- Entrez Gene: 10468 Human
- SwissProt: P19883 Human
Images
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Other - Human Follistatin ELISA Kit (ab192147)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Follistatin standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated values of Follistatin from a titration of Normal Human Serum (NHS) and Normal Human Plasma (EDTA) diluted within the working range of the assay and spiked with recombinant follistatin.(mean +/- SD, n =2).
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Interpolated values of Follistatin from HepG2 cell culture supernatants diluted within the working range of the assay (+/- SD, n =2). -
Quantitation of Follistatin expression in serum samples diluted to within working range from individual donors.Interpolated values of Follistatin are plotted on the bar graph. No signal was detected in Donors 6, 7 or 8.