Human FCGRT (FCRN) knockout HEK-293T cell line (ab265075)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Kidney -
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 11, 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 15, 20 TH01: 7, 9.3 TPOX: 11, 12 CSF1PO: 12 -
Antibiotic resistance
Puromycin 1.00µg/ml -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human FCGRT knockout HEK293T cell line (ab265075)All lanes : Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : FCGRT knockout A549 cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : Lncap cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaLanes 1-4: Merged signal (red and green). Green - ab228975 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab228975 Anti-FCGRT/FCRN antibody [EPR22627-25] was shown to specifically react with FCGRT/FCRN in wild-type A549 cells. Loss of signal was observed when knockout cell line ab265075 (knockout cell lysate ab257435) was used. Wild-type and FCGRT/FCRN knockout samples were subjected to SDS-PAGE. ab228975 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human FCGRT knockout HEK293T cell line (ab265075)Homozygous: 14 bp deletion in exon 3.
