Human FAP ELISA Kit (ab193701)
Key features and details
- Sensitivity: 12 pg/ml
- Range: 12 pg/ml - 4000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich
- Reacts with: Human
Overview
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Product name
Human FAP ELISA Kit
See all Fibroblast activation protein, alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich -
Sensitivity
12 pg/ml -
Range
12 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 91.52 84% - 100% Plasma 86.5 82% - 98% Cell culture media 91.09 83% - 99% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam's FAP Human ELISA Kit (ab193701) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FAP in serum, plasma, cell culture supernatants. (UniprotID: Q12884)
This assay employs an antibody specific for human FAP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures FAP present in the samples. The wells are washed and biotinylated anti-human FAP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of FAP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated Human FAP detection antibody (lyophilized) 2 vials Human FAP Standards (lyophilized) 2 vials Pre-coated Human FAP Microplate (12 Strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. -
Tissue specificity
Fibroblast specific. -
Sequence similarities
Belongs to the peptidase S9B family. -
Post-translational
modificationsN-glycosylated.
The N-terminus may be blocked. -
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. - Information by UniProt
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Alternative names
- 170 kDa melanoma membrane bound gelatinase
- 170 kDa melanoma membrane-bound gelatinase
- DPPIV
see all -
Database links
- Entrez Gene: 2191 Human
- Omim: 600403 Human
- SwissProt: Q12884 Human
- Unigene: 654370 Human
Images
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Example Standard Curve
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Example Standard Curve