Human FANCD2 knockout HeLa cell lysate (ab257173)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260096 - Human FANCD2 knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human FANCD2 knockout HeLa cell lysate (ab257173)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: FANCD2 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab178705 observed at 166 kDa. Red - loading control ab7291 observed at 50 kDa.
ab178705 Anti-FANCD2 antibody [EPR2279(3)] was shown to specifically react with FANCD2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261743 (knockout cell lysate ab257173) was used. Wild-type and FANCD2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab178705 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human FANCD2 knockout HeLa cell lysate (ab257173)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: FANCD2 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab108928 observed at 166 kDa. Red - loading control ab7291 observed at 50 kDa.
ab108928 Anti-FANCD2 antibody [EPR2302] was shown to specifically react with FANCD2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261743 (knockout cell lysate ab257173) was used. Wild-type and FANCD2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108928 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human FANCD2 knockout HeLa cell lysate (ab257173)Homozygous: 4 bp deletion in exon 13