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Human EphA2 Matched Antibody Pair Kit (ab219521)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 7.81 pg/ml - 500 pg/ml

Overview

  • Product name

    Human EphA2 Matched Antibody Pair Kit
    See all Eph receptor A2 kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Sensitivity

    2.26 pg/ml

  • Range

    7.81 pg/ml - 500 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human EphA2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human EphA2.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219051. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Human EphA2 Capture Antibody 2 x 50µg 1 x 50µg
    Human EphA2 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Human EphA2 Lyophilized Protein 2 vials 1 vial

  • Research areas

  • Function

    Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.

  • Tissue specificity

    Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.

  • Involvement in disease

    Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
    Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.

  • Post-translational
    modifications

    Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • ARCC2
    • AW545284
    • CTPA
    • CTPP1
    • CTRCT6
    • EC 2.7.10.1
    • Eck
    • Eph receptor A2
    • EPHA2
    • EPHA2_HUMAN
    • Ephrin receptor
    • Ephrin receptor EphA2
    • Ephrin type A receptor 2
    • Ephrin type-A receptor 2
    • Epithelial cell kinase
    • Epithelial cell receptor protein tyrosine kinase
    • Myk 2
    • Myk2
    • Sek 2
    • Sek2
    • Soluble EPHA2 variant 1
    • Tyrosine protein kinase receptor ECK
    • Tyrosine-protein kinase receptor ECK
    • Tyrosine-protein kinase receptor MPK-5
    • Tyrosine-protein kinase receptor SEK-2
    see all

  • Database links

    Images

    • Human EphA2 standard curve
      Human EphA2 standard curve

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Human EphA2 Matched Antibody Pair Kit (ab219521)
      Sandwich ELISA - Human EphA2 Matched Antibody Pair Kit (ab219521)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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