Human Eotaxin-2 ELISA Kit (ab226898)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.35 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Eotaxin-2 ELISA Kit
See all Eotaxin 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 9 1.5% Inter-assay Sample n Mean SD CV% Serum 3 1.95% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15.35 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 102% - 110% Serum 102 97% - 105% Hep Plasma 80 78% - 82% EDTA Plasma 102 94% - 107% Cit plasma 102 99% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Eotaxin-2 ELISA Kit (ab226898) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin-2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Eotaxin-2 with 15.35 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Eotaxin-2, also known as CCL24, MPIF-2 or Ckß-6, is a C-C chemokine originally identified in activated monocytes. Eotaxin-2 is expressed in activated T lymphocytes, GM-CSF treated macrophages and dermal fibroblasts. Secretion of Eotaxin-2 is known to recruit and activate CCR3-bearing cells particularly eosinophils as well as basophils. Activation of eosinophils generates reactive oxygen species, lipid mediators of inflammation and degranulation of toxic granule proteins. Chemotaxis and activation of basophils, previously primed with IL-3, lead to release of histamine and leukotriene C4.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml 10X Human Eotaxin-2 Capture Antibody 1 x 600µl 10X Human Eotaxin-2 Detector Antibody 1 x 600µl Human Eotaxin-2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3. -
Tissue specificity
Activated monocytes and activated T lymphocytes. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine 24
- C-C motif chemokine 24
- CCL24
see all -
Database links
- Entrez Gene: 6369 Human
- Omim: 602495 Human
- SwissProt: O00175 Human
- Unigene: 247838 Human
Images
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Other - Human Eotaxin-2 ELISA Kit (ab226898)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Eotaxin-2 Competitor standard curve comparisonStandard curve comparison between Human Eotaxin-2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of human Eotaxin-2 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Eotaxin-2 in human serum and plasma samplesThe concentrations of Eotaxin-2 were measured in duplicates, interpolated from the Eotaxin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 3.12%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin-2 concentration was determined to be 13,298 pg/mL in serum, 10,364 pg/mL in plasma (citrate), 9,354 pg/mL in plasma (EDTA), and 8,191 pg/mL in plasma (heparin).
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Interpolated concentrations of native Eotaxin-2 in human PBMC cell culture supernatantPBMCs were cultured in RPMI media and either stimulated with 1.5% PHA or unstimulated for 48 hours. The concentrations of Eotaxin-2 were measured in duplicate and interpolated from the Eotaxin-2 standard curve and corrected for sample dilution. Undiluted samples are as follows: PBMC stimulated 3.12% and PBMC unstimulated 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin-2 concentration was determined to be 107,776 pg/mL in stimulated PBMCs and 677 pg/mL in unstimulated PBMCs.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin-2 concentration was determined to be 13,607 pg/mL with a range of 4,760 – 35,609 pg/mL.
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Sandwich ELISA - Human Eotaxin-2 ELISA Kit (ab226898)To learn more about the advantages of recombinant antibodies see here.
