Human Cytokines FirePlex®-HT Panel 2 (ab234898)
Overview
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Product name
Human Cytokines FirePlex®-HT Panel 2 -
Sample type
Cell culture supernatant, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Multiplex -
Species reactivity
Reacts with: Human -
Product overview
Human Cytokines FirePlex®-HT Panel 2 (ab234898) uses FirePlex® particle technology to quantify IFN gamma, TNF alpha, IL10, IL-8, IL2, IL6 from 6.25 µl of sample input in 384-well plate format.
Assays are validated in serum, plasma, and cell culture supernatant, and can be run with 175 samples in duplicate per 384-well plate.
The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex-HT ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.
Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
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Notes
This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.
The following table provides the sensitivity, dynamic range, and CVs for FirePlex-HT Panel 2 - Human Cytokines kit*.
Target Sensitivity/LOD
(pg/ml)
Dynamic Range
(pg/ml)
Inter-well CV
(Std curve)
IFN gamma 72.48 123 - 10,000 11.5% IL-2 13.51 13.7 - 10,000 5.2% IL-6 3.06 4.57 - 10,000 8.4% IL-8 5.72 13.7 - 3,333 7.4% IL-10 22.75 41.1 - 10,000 9.0% TNF alpha 18.03 41.1 - 10,000 4.7% *representative dataset, not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.
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Platform
Multiplex
Properties
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Storage instructions
Please refer to protocols. -
Components Identifier 1 x 384 tests 1 units — — 384 Well Imaging Plate 1 x 384 tests 1 x 384 tests 384 Well Imaging Plate Lid 1 unit 1 unit 384 Well Imaging Plate Seal 2 units 2 units FirePlex-HT 1X PBS 1 x 8ml FirePlex-HT 5X Capture Particle Mix 1 x 700µl 1 x 700µl FirePlex-HT Human Protein Standard Mix HTA 1 x 7 tests 1 x 7 tests FirePlex-HT Reagent Diluent R1 1 x 8ml 1 x 8ml FirePlex-HT Sample Diluent S1 1 x 25ml 1 x 25ml FirePlex-HT - Imaging Dye Concentrate 1 x 875µl FirePlex-HT 35X Detector Antibody Mix 1 x 110µl 1 x 110µl -
Research areas
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Cellular localization
TNF alpha: Secreted and Cell membrane. IL-8: Secreted. IL-6: Secreted. IL-10: Secreted. Interferon gamma: Secreted. IL-2: Secreted. -
Database links
- Entrez Gene: 3458 Human
- Entrez Gene: 3558 Human
- Entrez Gene: 3569 Human
- Entrez Gene: 3576 Human
- Entrez Gene: 3586 Human
- Entrez Gene: 7124 Human
- Omim: 124092 Human
- Omim: 146930 Human
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Images
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FirePlex-HT Assay WorkflowFirePlex-HT Assay Workflow
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FirePlex-HT Panel 2 Standard CurveFirePlex-HT Panel 2 Standard Curve -
FirePlex-HT Analyte Dynamic RangeFirePlex-HT Analyte Dynamic Range
