Human Cytokine Antibody Array (200 Targets) - Quantitative (ab197428)
Overview
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Product name
Human Cytokine Antibody Array (200 Targets) - Quantitative -
Sample type
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate -
Assay type
Quantitative -
Assay time
6h 00m -
Species reactivity
Reacts with: Human -
Product overview
ab197428 can be used for quantitative measurement of 200 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Target names: BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-1δ, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNFα, TNFβ, TNF sRI, TNF sRII.
AR, BDNF, bFGF, BMP-4, BMP-5, BMP-7, β-NGF, EGF, EGF R, EG-VEGF, FGF-4, FGF-7, GDF-15, GDNF, GH, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, Insulin, MCSF R, NGF R, NT-3, NT-4, OPG, PDGF-AA, PIGF, SCF, SCF R, TGFα, TGFβ1, TGFβ3, VEGF, VEGF R2, VEGF R3, VEGF-D.
6Ckine, Axl, BTC, CCL28, CTACK, CXCL16, ENA-78, Eotaxin-3, GCP-2, GRO, HCC-1, HCC-4, IL-9, IL-17F, IL-18 BPa, IL-28A, IL-29, IL-31, IP-10, I-TAC, LIF, LIGHT, Lymphotactin, MCP-2, MCP-3, MCP-4, MDC, MIF, MIP-3α, MIP-3β, MPIF-1, MSPα, NAP-2, OPN, PARC, PF4, SDF-1α, TARC, TECK, TSLP.
4-1BB, ALCAM, B7-1, BCMA, CD14, CD30, CD40L, CEACAM-1, DR6, Dtk, Endoglin, ErbB3, E-Selectin, Fas, Flt-3L, GITR, HVEM, ICAM-3, IL-1 R4, IL-1 RI, IL-10 Rβ, IL-17R, IL-2Rγ, IL-21R, LIMPII, Lipocalin-2, L-Selectin, LYVE-1, MICA, MICB, NRG1-β1, PDGF Rβ, PECAM-1, RAGE, TIM-1, TRAIL R3, Trappin-2, uPAR, VCAM-1, XEDAR.
Activin A, AgRP, Angiogenin, ANG-1, Angiostatin, Cathepsin S, CD40, Cripto-1, DAN, DKK-1, E-Cadherin, EpCAM, Fas L, Fcγ RIIB/C, Follistatin, Galectin-7, ICAM-2, IL-13 R1, IL-13R2, IL-17B, IL-2 Rα, IL-2 Rβ, IL-23, LAP, NrCAM, PAI-1, PDGF-AB, Resistin, SDF-1β, sgp130, Shh N, Siglec-5, ST2, TGF-β2, Tie-2, TPO, TRAIL R4, TREM-1, VEGF-C, VEGF R1.
The analytes measured by this kit are split across five different arrays on different slides, all of which are provided in the kit.
Learn more about quantitive antibody arrays
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Notes
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.
Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array.Each antibody is spotted in quadruplicate on each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.
For high through-put, 4 slides can be nested into a tray matching a standard microplate (not supplied), allowing automated processing with a liquid handling workstation.
Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 8 tests 22 tests 50 tests 20X Wash Buffer I 8 x 30ml 15 x 30ml 30 x 30ml 20X Wash Buffer II 3 x 30ml 5 x 30ml 10 x 30ml Cy3 equivalent dye-conjugated Streptavidin 5 x 5µl 10 x 5µl 20 x 5µl Human Chemokine Array (40 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units Human Chemokine Array (40 T) Glass Slide 1 slide 2 slides 4 slides Human Chemokine Array (40 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 2 vials Human Cytokine Array A (40 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units Human Cytokine Array A (40 T) Glass Slide 1 slide 2 slides 4 slides Human Cytokine Array A (40 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 2 vials Human Growth Factor Array (40 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units Human Growth Factor Array (40 T) Glass Slide 1 slide 2 slides 4 slides Human Growth Factor Array (40 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 2 vials Human Inflammation Array (40 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units Human Inflammation Array (40 T) Glass Slide 1 slide 2 slides 4 slides Human Inflammation Array (40 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 2 vials Human Receptor Array (40 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units Human Receptor Array (40 T) Glass Slide 1 slide 2 slides 4 slides Human Receptor Array (40 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 2 vials Sample Diluent 3 x 15ml 5 x 15ml 10 x 15ml Slide Washer/Dryer (30 mL Centrifuge Tube) 3 units 5 units 10 units -
Research areas
Images
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Human Cytokine Antibody Array - Quantitative (200 Targets) (ab197428) - Assay Summary
Assay summary for Abcam Human Cytokine Antibody Array (200 Targets) - Quantitative (ab197428).