Human CXCL3 ELISA Kit (ab234574)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.1 pg/ml
- Range: 1.56 pg/ml - 100 pg/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CXCL3 ELISA Kit
See all GRO gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Protein 6 2.2% -
Sample type
Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
1.1 pg/ml -
Range
1.56 pg/ml - 100 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97 91% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CXCL3 ELISA Kit (ab234574) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL3 protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL3 with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CXCL3 Capture Antibody 1 x 600µl 10X Human CXCL3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CXCL3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 3
- C-X-C motif chemokine ligand 3
- Chemokine (C X C motif) ligand 3
see all -
Database links
- Entrez Gene: 2921 Human
- Omim: 139111 Human
- SwissProt: P19876 Human
- Unigene: 89690 Human
Images
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of CXCL3 were measured in duplicates, interpolated from the CXCL3 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 200.4 pg/mL in serum.
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To learn more about the advantages of recombinant antibodies see here.