Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human CTHRC1 ELISA Kit (ab274399)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 27.3 pg/ml
  • Range: 93.75 pg/ml - 6000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CTHRC1 ELISA Kit
    See all CTHRC1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 2.55%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 2.88%

  • Sample type

    Cell culture supernatant, Serum, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    27.3 pg/ml

  • Range

    93.75 pg/ml - 6000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100 99% - 101%
    Serum 97 91% - 101%
    Cit plasma 100 95% - 103%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human CTHRC1 ELISA kit (ab274399) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CTHRC1 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CTHRC1 with 27.3 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less


            -High sensitivity, specificity and reproducibility from superior antibodies


            -Fully validated in biological samples


            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Collagen triple helix repeat containing protein 1 (CTHRC1) is a 30 kDa secreted glycoprotein encoded by the CTHRC1 gene. CTHRC1 is known to regulate extracellular collagen deposition and partake in vascular remodeling of arterial injury as a function of cellular response through the TGF-β and Wnt pathways. Moreover, mutation of the gene has been associated with carcinogenesis in a variety of cell and tissues, most notably esophageal adenocarcinoma and Barrett’s esophagus. Furthermore, recent studies have demonstrated CTHRC1 as a marker of the severity of the disease progression in the dystrophic muscles.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of human CTHRC1 standard curve in Sample Diluent NS.
    Example of human CTHRC1 standard curve in Sample Diluent NS.

    The CTHRC1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native CTHRC1 in human serum, plasma and cell culture supernatant samples.
    Interpolated concentrations of native CTHRC1 in human serum, plasma and cell culture supernatant samples.

    The concentrations of CTHRC1 were measured in duplicates, interpolated from the CTHRC1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and SK-MEL28 cell culture supernatant 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTHRC1 concentration was determined to be 9,486 pg/mL in serum, 10,463 pg/mL in plasma (citrate) and 140,962 pg/mL in SK-MEL28 cell culture supernatant.

  • Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)
    Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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