Human CT-1 ELISA Kit (ab216166)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.2 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CT-1 ELISA Kit
See all CT-1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 3.2% Inter-assay Sample n Mean SD CV% Overall 5 7.2% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.2 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 115 104% - 122% Cell culture extracts 114 106% - 128% Tissue Extracts 109 102% - 121% Cell culture media 107 106% - 108% Hep Plasma 107 105% - 109% EDTA Plasma 109 106% - 116% Cit plasma 92 90% - 95% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CT-1 ELISA Kit (ab216166) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CT-1 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CT-1 with 3.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CT-1 (Cardiotrophin 1) is a secreted member of the IL-6 cytokine superfamily. It is a 201 amino acid protein which binds to and activates the ILST/gp130 heterodimer receptor. CT-1 induces cardiomyocyte hypertrophy in vitro and is a biomarker implicated in hypertension. It is highly expressed in heart, skeletal muscle, prostate and ovary. It has lower expression levels in lung, kidney, pancreas, thymus, testis and small intestine. There is little or no expression of CT-1 in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CT-1 Detector Antibody 1 x 600µl 10X Human CT-1 Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CT-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. -
Tissue specificity
Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cardiophin 1
- Cardiophin1
- Cardiotrophin I
see all -
Database links
- Entrez Gene: 1489 Human
- Omim: 600435 Human
- SwissProt: Q16619 Human
- Unigene: 483811 Human
Images
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Other - Human CT-1 ELISA Kit (ab216166)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Cardiotrophin 1 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Cardiotrophin 1 standard curve in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cardiotrophin 1 in human heart, skeletal muscle, and liver based on a 1,000 µg/mL extract load.The concentrations of Cardiotrophin 1 were measured in duplicate and interpolated from the Cardiotrophin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiotrophin 1 concentration was determined to be 1,029 pg/mL in heart, 1,550 pg/mL in skeletal muscle, and 78 pg/mL in liver.
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Interpolated concentrations of native Cardiotrophin 1 in PC-3 and U-87 MG cell extracts based on a 1,000 µg/mL extract load.The concentrations of Cardiotrophin 1 were measured in duplicate and interpolated from the Cardiotrophin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiotrophin 1 concentration was determined to be 429 pg/mL in PC-3, and 811 pg/mL in U-87 MG.
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Interpolated concentrations of spike Cardiotrophin 1 in human plasma samples.The concentrations of Cardiotrophin 1 were measured in duplicates, interpolated from the Cardiotrophin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100%, plasma (heparin) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spike Cardiotrophin 1 in human serum and cell culture media (RPMI + 10% FBS) samples. concentrations of spike Cardiotrophin 1 in human plasma samples.The concentrations of Cardiotrophin 1 were measured in duplicates, interpolated from the Cardiotrophin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and cell culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Other species reactivity was determined by measuring a 1,000 µg/mL extract load of various species heart tissue extract.The protein concentrations were interpolated from the human Cardiotrophin standard curve.
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Sandwich ELISA - Human CT-1 ELISA Kit (ab216166)To learn more about the advantages of recombinant antibodies see here.
