Human CST6 ELISA Kit (ab277411)
Key features and details
- Sensitivity: 0.14 ng/ml
- Range: 0.123 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CST6 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.14 ng/ml -
Range
0.123 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 118.9 83% - 143% Plasma 103.6 72% - 141% Cell culture media 100.5 82% - 129% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CST6 ELISA Kit (ab277411) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CST6 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CST6 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CST6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CST6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CST6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated Anti-Human CST6 Antibody 2 vials Anti-Human CST6 coated Microplate 1 unit Human CST6 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Shows moderate inhibition of cathepsin B but is not active against cathepsin C. -
Tissue specificity
Restricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands. Low expression levels are found in the nasal cavity. -
Sequence similarities
Belongs to the cystatin family. -
Post-translational
modificationsSubstrate for transglutaminases. Acts as an acyl acceptor but not as an acyl donor. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CST 6
- CST6
- Cystatin 6
see all -
Database links
- Entrez Gene: 1474 Human
- Omim: 601891 Human
- SwissProt: Q15828 Human
- Unigene: 139389 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.
