Human COX2 ELISA Kit (ab267646)
Key features and details
- Sensitivity: 1.2 ng/ml
- Range: 1.229 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human COX2 ELISA Kit
See all COX2 / Cyclooxygenase 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 ng/ml -
Range
1.229 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 133.8 114% - 149% Plasma 142.8 137% - 148% Cell culture media 108.3 86% - 129% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human COX2 ELISA Kit is designed for the quantitative determination of COX2 in cell culture supernatants, plasma and serum samples.
Δ Note: Human COX2 concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for human COX2 coated on a 96-well plate. Standards and samples are pipetted into the wells and COX2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human COX2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COX2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human COX2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human COX2 Detection Antibody 2 vials Human COX2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Alternative names
- COX 2
- COX-2
- COX2
see all -
Database links
- Entrez Gene: 5743 Human
- Omim: 600262 Human
- SwissProt: P35354 Human
- Unigene: 196384 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.