Human COPS7B (CSN7b) knockout HEK-293T cell line (ab266646)
Properties
-
Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Kidney -
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12 -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Purity
Immunogen affinity purified -
Research areas
Images
-
Western blot - Human COPS7B knockout HEK293T cell line (ab266646)All lanes : Anti-CSN7b antibody [EPR6465] (ab124718) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : COPS7B knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab124718 observed at 32 kDa. Red - loading control ab8245 observed at 36 kDa.
ab124718 Anti-CSN7b antibody [EPR6465] was shown to specifically react with CSN7b in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266646 (knockout cell lysate ab257895) was used. Wild-type and CSN7b knockout samples were subjected to SDS-PAGE. ab124718 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Human COPS7B knockout HEK293T cell line (ab266646)All lanes : Anti-CSN7b antibody [EPR6464] (ab133548) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : COPS7B knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab133548 observed at 32 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133548 Anti-CSN7b antibody [EPR6464] was shown to specifically react with CSN7b in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266646 (knockout cell lysate ab257895) was used. Wild-type and CSN7b knockout samples were subjected to SDS-PAGE. ab133548 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Sanger Sequencing - Human COPS7B knockout HEK293T cell line (ab266646)Homozygous: 1 bp insertion in exon3