Human Complement C2 ELISA Kit (ab154132)
Key features and details
- Sensitivity: 0.00323 µg/ml
- Range: 0.00323 µg/ml - 0.8 µg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C2 ELISA Kit
See all C2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.7% Inter-assay Sample n Mean SD CV% Overall 10.1% -
Sample type
Cell culture supernatant, Saliva, Milk, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.00323 µg/ml -
Range
0.003 µg/ml - 0.8 µg/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Rabbit, Dog, Pig -
Product overview
Abcam’s Complement C2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C2 in CSF, plasma, serum, saliva, and cell culture supernatants.
A Complement C2 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C2 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C2 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 70X Biotinylated Human Complement C2 Antibody 1 x 90µl Chromogen Substrate 1 x 8ml Complement C2 Microplate (12 x 8 well strips) 1 unit Complement C2 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. -
Involvement in disease
Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ARMD14
- C2
- C3/C5 convertase
see all -
Database links
- Entrez Gene: 717 Human
- Omim: 217000 Human
- SwissProt: P06681 Human
- Unigene: 408903 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab154132