Human Complement C1q ELISA Kit (ab170246)
Key features and details
- Sensitivity: 57 pg/ml
- Range: 0.313 ng/ml - 2.5 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C1q ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 4.9% Inter-assay Sample n Mean SD CV% Plasma 9.2% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
> 57 pg/ml -
Range
0.313 ng/ml - 2.5 ng/ml -
Recovery
= 98 %
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Assay time
4h 00m -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Rabbit, Cow, Pig -
Product overview
Complement C1q Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab170246) is designed for the quantitative measurement of Complement C1q concentrations in plasma, serum, saliva, urine, milk, cerebrospinal fluid and cell culture supernatants.
A Complement C1q specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C1q specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C1q captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C1q Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Complement C1q Microplate (12 x 8 well strips) 1 unit Complement C1q Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
Involvement in disease
Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C1qa
- C1QA_HUMAN
- C1QB
see all -
Database links
- Entrez Gene: 713 Human
- Entrez Gene: 714 Human
- Entrez Gene: 712 Human
- Omim: 120550 Human
- Omim: 120570 Human
- Omim: 120575 Human
- SwissProt: P02746 Human
- SwissProt: P02747 Human
see all
Images
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Typical Standard Curve
Representative Standard Curve using ab170246