Human CEBPB (CEBP Beta) knockout HeLa cell lysate (ab256874)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab261934 - Human CEBPB knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
-
Western blot - Human CEBPB knockout HeLa cell lysate (ab256874)
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: CEBPB knockout HeLa cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lanes 1-3: Merged signal (red and green). Green - ab32358 observed at 40 and 45 kDa. Red - loading control ab7291 observed at 50 kDa.
ab32358 Anti-CEBP Beta antibody [E299] - C-terminal was shown to specifically react with CEBP Beta in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261771 (knockout cell lysate ab256874) was used. Wild-type and CEBP Beta knockout samples were subjected to SDS-PAGE. ab32358 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Sanger Sequencing - Human CEBPB knockout HeLa cell lysate (ab256874)Allele-1: 11 bp deletion in exon1
-
Sanger Sequencing - Human CEBPB knockout HeLa cell lysate (ab256874)Allele-2: 1 bp deletion in exon1
