Human CD69 ELISA Kit (ab272464)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 25.97 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture media, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD69 ELISA Kit
See all CD69 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3.3% Inter-assay Sample n Mean SD CV% Extract 3 3.4% -
Sample type
Serum, Tissue Extracts, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
25.97 pg/ml -
Range
93.75 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103 100% - 106% Tissue Extracts 108 102% - 115% Cell culture media 85 81% - 91% Hep Plasma 98 86% - 110% EDTA Plasma 90 86% - 93% Cit plasma 110 99% - 116% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD69 ELISA kit (ab272464) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neurofilament H protein in serum, plasma, cell culture supernatant and cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD69 with 25.97 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD69 is an early activation marker for hematopoietic stem cells. It is expressed on activated T-cells, B-cells, NK cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. CD69 is involved in lymphocyte proliferation and signal transduction.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD69 Capture Antibody 1 x 600µl 10X Human CD69 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CD69 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. -
Tissue specificity
Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
Earliest inducible cell surface glycoprotein acquired during lymphoid activation. -
Post-translational
modificationsConstitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Activation inducer molecule
- Activation inducer molecule (AIM/CD69)
- AIM
see all -
Database links
- Entrez Gene: 969 Human
- Omim: 107273 Human
- SwissProt: Q07108 Human
- Unigene: 208854 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD69 standard curve in Sample Diluent NS.The CD69 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD69 in human serum and plasma (heparin) samples.The concentrations of CD69 were measured in duplicates, interpolated from the CD69 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD69 concentration was determined to be 2,875.88 pg/mL in serum and 2,157.97 pg/mL in plasma (heparin).
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Interpolated concentrations of native CD69 in human U937 cell extract based on a 375 µg/mL extract load.The concentrations of CD69 were measured in duplicate and interpolated from the CD69 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD69 concentration was determined to be 3,432.14 pg/mL in U937 cell extract.
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Interpolated concentrations of native CD69 in stimulated and unstimulated human Daudi cell extract based on a 250 µg/mL extract load.Stimulated Daudi cell extract samples were treated with 50 ng/mL PMA for 24 hours. The concentrations of CD69 were measured in duplicate and interpolated from the CD69 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD69 concentration was determined to be 6,406.69 pg/mL in PMA stimulated Daudi cell extract and non-detectable in unstimulated Daudi cell extract.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD69 concentration was determined to be 6.44 ng/mL with a range of 0.31 – 49.92 ng/mL.
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Sandwich ELISA - Human CD69 ELISA Kit (ab272464)To learn more about the advantages of recombinant antibodies see here.
