Human CD35 ELISA Kit (ab277439)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD35 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.205 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99.34 96% - 104% Plasma 91.54 88% - 97% Cell culture media 96.65 89% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD35 ELISA Kit (ab277439) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD35 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CD35 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CD35 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CD35 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CD35 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 900x HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human CD35 Antibody 2 vials Anti-Human CD35 coated Microplate 1 unit Human CD35 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Mediates cellular binding of particles and immune complexes that have activated complement. -
Tissue specificity
Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- C3 binding protein
- C3b/C4b receptor
- C3BR
see all -
Database links
- Entrez Gene: 1378 Human
- Omim: 120620 Human
- SwissProt: P17927 Human
- Unigene: 334019 Human
Images
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Human CD35 ELISA kit (ab277439) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.