Human CCL27 ELISA Kit (CTACK) (ab200013)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9 pg/ml
- Range: 94 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CCL27 ELISA Kit (CTACK)
See all CCL27 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 8.7% Inter-assay Sample n Mean SD CV% Overall 8 7.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
9 pg/ml -
Range
94 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 113 104% - 129% Cell culture media 89 86% - 94% Hep Plasma 85 73% - 98% EDTA Plasma 89 86% - 94% Cit plasma 113 109% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Goat, Cow, Pig -
Product overview
Human CCL27 ELISA Kit (CTACK) (ab200013) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL27 (CTACK) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL27 (CTACK) with 9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CC chemokine ligand 27 (CCL27) is a member of a superfamily of small secreted proteins that attract circulating lymphocytes to an area of injury/infection by binding with G protein-coupled receptors expressed on the migrating target cells. CCL27 is expressed in the skin and selectively attracts CLA+ memory T-cells into the skin microenvironment. CCL27 expression can be up-regulated by the proinflammatory cytokines TNF-α and IL-1β increasing migration of memory T-cells to the skin during inflammation. In normal skin suprabasal keratinocytes express minute amounts of CCL27; where as in lesions of human Atopic Dermatitis, Contact Dermatitis, and psoriasis, the keratinocytes demonstrate stronger expression of CCL27 which is thought to attract pro-inflammatory CLA+ memory T-cells to the lesions contributing to the inflammatory process and perpetuates the disease.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CCL27 Capture Antibody 1 x 600µl 10X Human CCL27 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human CCL27 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10. -
Tissue specificity
Testis, thymus, placenta, ovary and skin. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ALP
- C-C motif chemokine 27
- CC chemokine ILC
see all -
Database links
- Entrez Gene: 10850 Human
- Omim: 604833 Human
- SwissProt: Q9Y4X3 Human
- Unigene: 648124 Human
Images
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Other - Human CCL27 ELISA Kit (CTACK) (ab200013)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of CCL27 standard curve prepared in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of standard curve prepared in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of Human serum and plasma (EDTA & citrate) to within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Comparison of CCL27 expression in individual donor samples.Background subtracted data from duplicate measurements are plotted. Serum samples from a total of 10 individual Normal serum donors and 3 donors diagnosed with Atopic Dermatitis (AD) were assayed. Complete medical histories for all donors is not known.
