Human CASP8 (Caspase-8) knockout HeLa cell lysate (ab256857)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab261930 - Human CASP8 knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
-
Western blot - Human CASP8 (Caspase-8) knockout HeLa cell lysate (ab256857)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: CASP8 knockout HeLa cell lysate (20µg)
Lane 3: Jurkat cell lysate (20µg)
Lane 4: SH-SY5Y cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab32397 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab32397 Rabbit monoclonal [EPR2418Y] to IRF3 was shown to specifically react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human CASP8 (Caspase-8) knockout HeLa cell lysate (ab256857)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: CASP8 knockout HeLa cell lysate (20µg)
Lane 3: Jurkat cell lysate (20µg)
Lane 4: SH-SY5Y cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab32125 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab32125 Anti-Caspase-8 antibody [E6] was shown to specifically react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 3000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human CASP8 knockout HeLa cell lysate (ab256857)Homozygous: 2 bp deletion in exon 3
