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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly

Human BRK1 (C3orf10) knockout HeLa cell lysate (ab258794)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human BRK1 (C3orf10) knockout HeLa cell lysate (ab258794)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human BRK1 (C3orf10) knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 4 bp deletion in exon1 and 88 bp insertion in exon1.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab262543 - Human BRK1 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Target

  • Relevance

    C3orf10 is involved in regulation of actin and microtubule organization and is part of a WAVE complex that activates the Arp2/3 complex.
  • Cellular localization

    Cytoplasm, cytoskeleton
  • Alternative names

    • BRICK1, SCAR/WAVE actin nucleating complex subunit
    • BRICK1, SCAR/WAVE actin nucleating complex subunit, homolog
    • BRK1
    • Brk1-like
    • C3orf10 chromosome 3 open reading frame 10
    • C3ORF10 GENE
    • Chromosome 3 open reading frame 10
    • Haematopoietic stem/progenitor cell protein 300
    • hHBrk1
    • HSPC300
    • MDS027
    • Probable protein BRICK1
    • Protein BRICK1
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab262543 - Human BRK1 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Images

  • Sanger Sequencing - Human BRK1 knockout HeLa cell lysate (ab258794)
    Sanger Sequencing - Human BRK1 knockout HeLa cell lysate (ab258794)

    Allele-1: 4 bp deletion in exon1

     

  • Sanger Sequencing - Human BRK1 knockout HeLa cell lysate (ab258794)
    Sanger Sequencing - Human BRK1 knockout HeLa cell lysate (ab258794)

    Allele-2: 1 bp insertion in exon1

     

  • Sanger Sequencing - Human BRK1 knockout HeLa cell lysate (ab258794)
    Sanger Sequencing - Human BRK1 knockout HeLa cell lysate (ab258794)

    Allele-3: 88 bp insertion in exon1

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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