Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human BMP2 ELISA Kit (ab277085)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 79.8 pg/ml
  • Range: 187.5 pg/ml - 12000 pg/ml
  • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human BMP2 ELISA Kit
    See all BMP2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 2.7%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.2%

  • Sample type

    Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    79.8 pg/ml

  • Range

    187.5 pg/ml - 12000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 97 92% - 107%
    Serum 101 99% - 105%
    Cell culture media 99 96% - 102%
    Hep Plasma 95 90% - 101%
    EDTA Plasma 107 97% - 107%
    Cit plasma 95 91% - 102%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human BMP2 ELISA kit (ab277085) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human BMP2 protein in human serum, plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BMP2 with 79.8 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Bone morphogenetic protein-2 (BMP2, BMP 2A) belongs to the transforming growth factor beta (TGF-β) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP2 has been identified as a candidate mediator of retinoid activity. BMP2 protein induces medulloblastoma cell apoptosis, whereas the BMP2 antagonist noggin blocks both retinoid and BMP2-induced apoptosis. BMP2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of human BMP2 standard curve in Sample Diluent NS.
    Example of human BMP2 standard curve in Sample Diluent NS.

    The BMP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • . Interpolated concentrations of spike BMP2 in human serum, plasma and cell culture media samples.
    . Interpolated concentrations of spike BMP2 in human serum, plasma and cell culture media samples.

    The concentrations of BMP2 were measured in duplicates, interpolated from the BMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25% and cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Human BMP2 ELISA Kit
    Sandwich ELISA - Human BMP2 ELISA Kit

    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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