Human Anti-Helicobacter pylori IgG ELISA Kit (ab108736)
Key features and details
- Sensitivity: 3 U/ml
- Range: 3 U/ml - 150 U/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
-
Product name
Human Anti-Helicobacter pylori IgG ELISA Kit
See all Helicobacter pylori IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos.Serum 19 6.1% Pos.Serum 19 7% Inter-assay Sample n Mean SD CV% Pos.Serum 13 7.2% Pos.Serum 13 3% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
3 U/ml -
Range
3 U/ml - 150 U/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Helicobacter pylori IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Helicobacter pylori in Human serum and plasma.
A 96-well plate has been precoated with Helicobacter pylori antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Helicobacter pylori-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Helicobacter pylori IgG sample captured in plate.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Helicobacter pylori (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Helicobacter pylori anti-IgG HRP conjugate Blue, Black cap 1 x 20ml Helicobacter pylori IgG Standard A – 0 U/mL Blue Cap 1 x 2ml Helicobacter pylori IgG Standard B – 15 U/mL Green Cap 1 x 2ml Helicobacter pylori IgG Standard C – 75 U/mL Yellow cap 1 x 2ml Helicobacter pylori IgG Standard D – 150 U/mL Red cap 1 x 2ml IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
-
Typical Standard Curve
Representative Standard Curve using ab108736
