Human Anti-Corynebacterium diphtheriae toxin IgG ELISA Kit (ab108761)
Key features and details
- Sensitivity: 0.01 U/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-Corynebacterium diphtheriae toxin IgG ELISA Kit
See all Corynebacterium diphtheriae toxin IgG kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma -
Assay type
Indirect -
Sensitivity
0.01 U/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Corynebacterium diphtheriae toxin plus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Corynebacterium diphtheriae toxin in Human serum and plasma.
A 96-well plate has been precoated with Corynebacterium diphtheriae toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Corynebacterium diphtheriae toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Corynebacterium diphtheriae toxin IgG sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Corynebacterium diphtheriae toxin (IgG) Coated Microplate (12 x 8 wells) 12 x 8 tests Corynebacterium diphtheriae toxin anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Corynebacterium diphtheriae toxin IgG Additional Control Yellow, Orange cap 1 x 2ml Corynebacterium diphtheriae toxin IgG Standard A - 0.000 U/mL Yellow, Blue cap 1 x 2ml Corynebacterium diphtheriae toxin IgG Standard B - 0.015 U/mL Yellow, Green Cap 1 x 2ml Corynebacterium diphtheriae toxin IgG Standard C - 0.075 U/mL Yellow, Yellow cap 1 x 2ml Corynebacterium diphtheriae toxin IgG Standard D - 0.150 U/mL Yellow, Red cap 1 x 2ml Corynebacterium diphtheriae toxin IgG Standard E - 1.000 U/mL Yellow, White cap 1 x 2ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution Red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
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Alternative names
- C. diphtheriae toxin 5S IgG
- C. diphtheriae toxin IgG
- Diphtheria toxin 5S IgG
Images
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Typical Standard Curve
Representative Standard Curve using ab108761
