Human ALDH3A2 (Aldehyde dehydrogenase 10) knockout HeLa cell line (ab265427)
Properties
-
Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Cervix -
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10 -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% DMSO, 2% Cellulose, methyl ether -
Research areas
Images
-
Western blot - Human ALDH3A2 (Aldehyde dehydrogenase 10) knockout HeLa cell line (ab265427)All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ALDH3A2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Lanes 1 - 2:Merged signal (red and green). Green - ab184171 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab184171 was shown to react with ALDH3A2 in wild-type HeLa cells in Western blot with loss of signal observed in ALDH3A2 knockout cell line ab265427 (ALDH3A2 knockout cell lysate ab257829). Wild-type HeLa and ALDH3A2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab184171 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging. -
Sanger Sequencing - Human ALDH3A2 knockout HeLa cell line (ab265427)Homozygous: Insertion of the selection cassette in exon 1.