Human ADAMTS1 ELISA Kit (ab213751)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 93.8 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human ADAMTS1 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human ADAMTS1 antibody 1 x 130µl Lyophilized recombinant Human ADAMTS1 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 1
- ADAM-TS 1
- ADAM-TS1
see all -
Database links
- Entrez Gene: 9510 Human
- Entrez Gene: 9510 Human
- Omim: 605174 Human
- SwissProt: Q9UHI8 Human
- SwissProt: Q9UHI8 Human
- Unigene: 643357 Human
- Unigene: 643357 Human
Images
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Human ADAMTS1 ELISA Kit (ab213751) Standard Curve.
Human ADAMTS1 ELISA Kit (ab213751) Standard Curve.