Human ACE ELISA Kit (ab263889)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.15 ng/ml
- Range: 0.625 ng/ml - 40 ng/ml
- Sample type: Cell culture media, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ACE ELISA Kit
See all Angiotensin Converting Enzyme 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.4% Inter-assay Sample n Mean SD CV% Serum 3 3% -
Sample type
Serum, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.15 ng/ml -
Range
0.625 ng/ml - 40 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 87 78% - 96% Cell culture media 82 80% - 85% Hep Plasma 87 80% - 94% EDTA Plasma 104 102% - 109% Cit plasma 86 84% - 86% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human ACE ELISA Kit (ab263889) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACE protein in cell culture media, cell lysate, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ACE with 0.15 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ACE (Angiotensin-converting enzyme) is an enzyme that converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. It is present as plasma membrane and soluble forms. ACE is also able to inactivate bradykinin, a potent vasodilator. ACE has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ACE Capture Antibody 1 x 600µl 10X Human ACE Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human ACE Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. -
Tissue specificity
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. -
Involvement in disease
Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsPhosphorylated by CK2 on Ser-1299; which allows membrane retention. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- ACE
- ACE 1
- ACE T
see all -
Database links
- Entrez Gene: 1636 Human
- Omim: 106180 Human
- SwissProt: P12821 Human
- Unigene: 298469 Human
Images
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Human ACE standard curve comparison
Standard Curve comparison between human ACE SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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SimpleStep ELISA Protocol DiagramSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ACE standard curve in Sample Diluent NS.The ACE standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ACE in human serum and plasma samples.The concentrations of ACE were measured in duplicates, interpolated from the ACE standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 15%, plasma (EDTA) 7.5% and plasma (heparin) 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE concentration was determined to be 303 ng/mL in neat serum, 259 ng/mL in neat plasma (citrate), 312 ng/mL in neat plasma (EDTA) and 245 ng/mL in neat plasma (heparin).
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Interpolated concentrations of native ACE in human lung tissue extract sample based on a 125 µg/mL extract load.The concentrations of ACE were measured in duplicate and interpolated from the ACE standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE concentration was determined to be 30 ng/mL in sample.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE concentration was determined to be 273 ng/mL with a range of 200 – 418 ng/mL.
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Sandwich ELISA - Human ACE ELISA Kit (ab263889)To learn more about the advantages of recombinant antibodies see here.
