HRP Anti-M13 antibody [B62-FE2] (ab50370)
![HRP Anti-M13 antibody [B62-FE2] (ab50370)](https://www.abcam.com/ps/products/50/ab50370/Images/ab50370-274541-anti-m13-antibody-b62-fe2-hrp-elisa.jpg "HRP Anti-M13 antibody [B62-FE2] (ab50370)")
Key features and details
- HRP Mouse monoclonal [B62-FE2] to M13
- Suitable for: ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-M13 antibody [B62-FE2]
See all M13 primary antibodies -
Description
HRP Mouse monoclonal [B62-FE2] to M13 -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
ab50370 was raised using fd phages from E. Coli F+ strain (JM109).
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Epitope
ab50370 binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT. -
General notes
Strongly recommend adding 0.5% BSA to diluents and a suitable preservative (as 0.05-0.09% sodium azide or equivalent) for extra stablity when stored at 2-8 °C
Filamentous fd bacteriophages are used in immunoassays for the identification of recombinant antigen- or antibody phages. They have also been used to construct phage-display peptide libraries, which have been useful in selecting peptides that interact with specific domains within target molecules.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
Filamentous fd bacteriophages are used in immunoassays for the identification of recombinant antigen- or antibody phages. They have also been used to construct phage-display peptide libraries, which have been useful in selecting peptides that interact with specific domains within target molecules. -
Clonality
Monoclonal -
Clone number
B62-FE2 -
Isotype
IgG2b -
Research areas
Images
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ELISA - HRP Anti-M13 antibody [B62-FE2] (ab50370) This image is courtesy of an anonymous Abreview
ab50370 detecting M13 phage. Samples were blocked with 1% BSA for 2 hours at 25°C and incubated with the primary antibody (1/1000 in 50mM carbonate/bicarbonate buffer, pH 9) for 12 hours at 4°C.
