HRP Anti-Cleaved PARP1 antibody [Y34] (ab237434)
![HRP Anti-Cleaved PARP1 antibody [Y34] (ab237434)](https://www.abcam.com/ps/products/237/ab237434/Images/ab237434-344424-237434WB1jpg.jpg "HRP Anti-Cleaved PARP1 antibody [Y34] (ab237434)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y34] to Cleaved PARP1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-Cleaved PARP1 antibody [Y34]
See all Cleaved PARP1 primary antibodies -
Description
HRP Rabbit monoclonal [Y34] to Cleaved PARP1 -
Host species
Rabbit -
Conjugation
HRP -
Specificity
This antibody is specific for p85 cleaved form of PARP1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HAP1 (WT) staurosporine treated.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y34 -
Isotype
IgG -
Research areas
Images
-
Western blot - HRP Anti-Cleaved PARP1 antibody [Y34] (ab237434)
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 50 min. before being transferred onto a nitrocellulose membrane at 30V for 70 min. The membrane was then blocked for an hour using 3% non-fat milk before being incubated with ab237434 (1/1000) overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Samples:
Lane 1. HAP1 (WT) staurosporine treated (20 µg)
Lane 2. HAP1 PARP1 (KO) staurosporine treated (20 µg)
-
![HRP Anti-Cleaved PARP1 antibody [Y34] (ab237434)](https://www.abcam.com/ps/products/237/ab237434/Images/ab237434-2-benefits-of-recombinant-antibodies.png)
HRP Anti-Cleaved PARP1 antibody [Y34] (ab237434)