Horse MMP9 ELISA kit (ab272029)
Key features and details
- Sensitivity: 49 pg/ml
- Range: 49.15 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse, Human
Overview
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Product name
Horse MMP9 ELISA kit
See all MMP9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
49 pg/ml -
Range
49.15 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 118.1 95% - 147% Serum 112.5 92% - 129% Plasma 100.4 84% - 116% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Horse, Human -
Product overview
Horse MMP-9 ELISA Kit is designed for the quantitative determination of Horse MMP-9 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for MMP-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse MMP-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Detects horse and human MMP9.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Horse MMP9 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Horse MMP9 Detection Antibody 2 vials Horse MMP9 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
Tissue specificity
Produced by normal alveolar macrophages and granulocytes. -
Involvement in disease
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Alternative names
- 82 kDa matrix metalloproteinase-9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
see all -
Database links
- Entrez Gene: 4318 Human
- Omim: 120361 Human
- SwissProt: P14780 Human
- Unigene: 297413 Human
Images
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Horse MMP-9 Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
Horse MMP-9 ELISA kit (ab272029) Standard curve