Horse IL-2 ELISA kit (ab272027)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.22 ng/ml - 160 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse
Overview
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Product name
Horse IL-2 ELISA kit
See all IL-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.22 ng/ml - 160 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 81.71 69% - 96% Serum 83.62 68% - 103% Plasma 79.81 69% - 92% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Horse -
Product overview
Horse IL-2 ELISA kit is designed for the quantitative determination of Equine IL-2 in cell culture supernatants, plasma and serum samples.
ΔNote: (Equine IL-2 concentration is low in normal serum/plasma and may not be detectable in this assay
This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Equine IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This kit detects equine IL-2.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Horse IL-2 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Horse IL-2 Detection Antibody 2 vials Horse IL-2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all
Images
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Equine IL-2 Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
Equine IL-2 ELISA kit (ab272027) Standard curve.