HLA-DP overexpression 293T lysate (whole cell) (ab94190)
Overview
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Product name
HLA-DP overexpression 293T lysate (whole cell) -
General notes
ab94190 is a 293T cell transfected lysate in which Human HLA DP has been transiently over-expressed using a pCMV-HLS DP plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.
Images
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SDS-PAGE - HLA-DP overexpression 293T lysate (whole cell) (ab94190)ab94190 at 15 μg/lane on an SDS-PAGE gel
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Western blot - HLA-DP overexpression 293T lysate (whole cell) (ab94190)All lanes : Anti-HLA-DP antibody (ab88089) at 1/500 dilution
Lane 1 :HLA-DP overexpression 293T lysate (whole cell) (ab94190)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
