HIV-1 Protease Inhibitor Assay Kit (Fluorometric) (ab211106)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
HIV-1 Protease Inhibitor Assay Kit (Fluorometric)
See all HIV1 Protease kits -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Semi-quantitative -
Product overview
HIV-1 Protease Inhibitor Screening Kit (Fluorometric) (ab211106) provides a rapid, simple test suitable for high-throughput screening of HIV-1 Protease inhibitors. The assay is based on the ability of active HIV-1 protease to cleave a synthetic peptide substrate to release a free fluorophore, which can be easily quantified using a fluorescence microplate reader. In the presence of a HIV-1 Protease inhibitor, the cleavage of the substrate is reduced/abolished, resulting in decrease or total loss of the fluorescence.
This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HIV-1 Protease.
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Notes
Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV-1 protease, HIV virions remain non-infectious.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 100 tests HIV-1 Protease 2 vials HIV-1 Protease Assay Buffer 1 x 25ml HIV-1 Protease Dilution Buffer 1 x 1ml HIV-1 Protease Inhibitor (1 mM Pepstatin A in DMSO) 1 x 20µl HIV-1 Protease Substrate 1 x 200µl -
Research areas
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Alternative names
- HIV-1 protease
Images
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HIV-1 Protease Inhibitor Screening Kit (Fluorometric) (ab211106)
Typical inhibition curve of HIV-1 Protease activity by the protease inhibitor Pepstatin A (IC50 = 1.6 µM) Assay was performed following the kit protocol.