HIV-1 Protease Activity Assay Kit (Fluorometric) (ab211105)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Purified protein, Tissue Lysate
Overview
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Product name
HIV-1 Protease Activity Assay Kit (Fluorometric)
See all HIV1 Protease kits -
Detection method
Fluorescent -
Sample type
Purified protein, Tissue Lysate -
Assay type
Quantitative -
Product overview
HIV-1 Protease Activity Assay Kit (Fluorometric) (ab211105) provides a convenient and simple method for detecting the activity of recombinant and/or purified HIV-1 protease. The assay kit is based on the ability of active HIV-1 protease to cleave a synthetic peptide substrate to release the free fluorophore which can be easily quantified at Ex/Em = 330/450 nm using a fluorescence microplate reader.
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Notes
Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV1 protease, HIV virions remain non-infectious.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 100 tests Fl. Standard (10 mM in DMSO) 1 x 20µl HIV-1 Protease (Positive Control) 1 x 7µl HIV-1 Protease Assay Buffer 1 x 25ml HIV-1 Protease Dilution Buffer 1 x 1ml HIV-1 Protease Substrate 1 x 200µl -
Research areas
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Alternative names
- HIV-1 protease
Images
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HIV-1 Protease Activity Assay Kit (Fluorometric) (ab211105)
Typical Fl. standard calibration curve.
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HIV-1 Protease Activity Assay Kit (Fluorometric) (ab211105)Kinetic progress curves observed for increasing amounts of HIV-1 Protease (positive control, included in the kit) through time.
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HIV-1 Protease Activity Assay Kit (Fluorometric) (ab211105)Measurement of HIV-1 Protease activity using increasing HIV-1 Protease positive control included in the kit, calculated from data from kinetic curves.
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HIV-1 Protease Activity Assay Kit (Fluorometric) (ab211105)Measurement of HIV-1 Protease activity using HIV-1 Protease included in kit as positive control and activity of HIV-1 Protease in presence of Pepstatin A (protease inhibitor).