Hela-Vehicle treated (Negative Control) Whole Cell Lysate (ab116321)
Overview
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Product name
Hela-Vehicle treated (Negative Control) Whole Cell Lysate -
General notes
We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.
Product was previously marketed under the MitoSciences sub-brand.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 1.8% Sodium lauryl sulfate, 10% Glycerol, 0.0002% Bromophenol blue, 1.54% DTT, 0.7% Sodium chloride, 1.1% Tris, 0.3% Sodium fluoride
This Lysate was normalised with SDS-PAGE sample buffer to current concentration. -
Concentration information loading... -
Background
HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
Images
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SDS-PAGE - Hela-Vehicle treated (Negative Control) Whole Cell Lysate (ab116321)
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Western blot - Hela-Vehicle treated (Negative Control) Whole Cell Lysate (ab116321)All lanes : Anti-HIF-1 alpha antibody (ab103063) at 2 µg/ml
Lane 1 :Hela-Vehicle treated (Negative Control) Whole Cell Lysate (ab116321)
Lane 2 : Hela-DFO treated (0.5mM, 24h) Whole Cell Lysate (ab116322)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Ab103063 was diluted in 1% milk/PBS. Anti-Rabbit Secondary antibody was diluted in 2% milk/PBS.
