HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (ab135395)
Overview
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Product name
HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated -
Product overview
HeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media. The protein content was determined by BCA assay. Calyculin A is a potent phosphatase inhibitor. Unlike Okadaic Acid, which only acts on protein phosphatase PP2A, Calyculin A inhibits both PP1 and PP2A. Cells treated with Calyculin A accumulate serine/threonine phosphorylation making them a strong positive control for phospho-threonine or phospho-serine specific antibodies.
Concentration:
HeLa Lysate - Vehicle Treated Control - 200 µg at 1 mg/mL
HeLa Lysate - Calyculin Treated - 200 µg at 1 mg/mLThe lysate is supplied in SDS-PAGE loading buffer with DTT and is ready to load on a gel.
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Tested applications
Suitable for: WB, SDS-PAGEmore details
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 2 units Calyculin-Treated HeLa Lysate 1 x 200µg Control for Calyculin-Treated HeLa Lysate 1 x 200µg -
Research areas
Images
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Western Blot
All lanes: Anti-Phosphoserine/Threonine antibody (ab17464) at 1/1000
Lane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3: HeLa Whole Cell Lysate – Untreated Control at 20 µg
Secondary:
Goat polyclonal to Rabbit IgG – Pre-Adsorbed (HRP) at 1/6000 developed using the ECL technique.
Performed under reducing conditions. -
SDS-PAGELane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3: HeLa Whole Cell Lysate – Untreated control at 20 µg
Gel
10-20% acrylamide separation gel