Guinea Pig IgG ELISA Kit (ab190813)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13 pg/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Guinea pig
Overview
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Product name
Guinea Pig IgG ELISA Kit
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Guin Pig IgG 3 3.49% Inter-assay Sample n Mean SD CV% Guin Pig IgG 3 6.17% -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
13 pg/ml -
Range
0.16 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Antibody Diluent 4B 101.27 94.27% - 100.98% 1X Cell Extraction Buffer PTR 89.19 85.67% - 101.36% 1X Wash Buffer 95.45 90.23% - 100.98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Guinea pig
Does not react with: Goat, Cow, Pig -
Product overview
Guinea Pig IgG ELISA Kit (ab190813) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pig IgG protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Guinea Pig IgG with 13 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
There are four classes of immunoglobulins in Guinea Pig: IgM, IgE, IgD and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In Guinea Pig, the IgG class is further divided into two isotypes: IgG1 and IgG2. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane. IgG is involved in response to a foreign antigen. The presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, it can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically Guinea Pig serum and plasma samples contain about 7 to 10 mg/ml of IgG.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Guinea Pig IgG Capture Antibody 1 x 600µl 10X Guinea Pig IgG Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4B 1 x 6ml Guinea Pig IgG Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
Images
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Other - Guinea Pig IgG ELISA Kit (ab190813)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Guinea Pig IgG standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Example of Guinea Pig serum IgG level in Sample Diluent NSInterpolated Guinea Pig IgG value are graphed.
