Gold-Hydrazide Conjugation Kit (40 nm, 10 OD) (ab188284)
Overview
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Product name
Gold-Hydrazide Conjugation Kit (40 nm, 10 OD)
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Product overview
Antibodies can be covalently attached quickly and easily, in a site-specific fashion, to ultra-stable Gold nanoparticles functionalized with hydrazides. The Abcam Gold nanoparticles coated with a special surface material which remains firmly anchored even under the most extreme conditions. Gold derivatives display unrivalled colloidal stability. The hands-on time for the Gold conjugation procedure is about 1 minute and the conjugate is ready to use within 30 minutes.
While antibodies in their native state do not contain aldehyde groups, it is easy to introduce aldehydes by mild oxidation of the sugar chains located in the Fc domain. Covalent attachment of aldehyde-modified antibodies to hydrazide surfaces therefore attaches the antibody via the Fc region and orients the two antigen-binding arms in such a way as to maximize antigen binding, which can lead to enhanced assay performance.
Gold-Hydrazide Conjugation Kit (40 nm, 10 OD) (ab188284) provides all the materials required to conjugate three antibodies in site specific manner to Gold nanoparticles. Sufficient material is provided for small scale trial conjugations and then scale up of the best reagent.
Benefits of Gold-Hydrazide Conjugation Kit (40 nm, 10 OD):
Site-specific labelling – Antigen binding sites of antibodies are free to bind the target molecule
Proprietary surface coating prevents metal-protein interactions, and enables covalent attachment to the Gold – Stable conjugates formed
Fully scalable – Easy transfer from R&D to manufacturing
Uniform spherical shape and narrow size distribution – Consistent high quality and excellent batch-to-batch reproducibility
Buffer requirements:
We recommend the antibody to be in a suitable buffer to allow aldehyde groups to be introduced into the Fc domain, pH 7. This buffer can thus contain 100 m M phosphate, non-buffering salts (e.g. sodium chloride), chelating agents (e.g. EDTA). For incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits for Nanoparticles. To learn more about incompatible buffers, please refer to the protocol booklet.
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Notes
This product is manufactured by Expedeon, an Abcam company, and was previously called 40nm InnovaCoat® GOLD - Hydrazide. 280-0100 is the same as the 30 µg size (1 test). 280-0000 is the same as the 3 x 30 µg size (3 tests). 280-0300 is the same as the 90 µg size (1 test).
The 30 µg and 3 x 30 µg Conjugation Kits are designed to label 10-30 µg of antibody per vial, and the 90 µg Conjugation Kits are designed to label 30-90 µg of antibody per vial, the optimum amount of antibody being application dependent.
Properties
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Storage instructions
Please refer to protocols. -
Components 90 µg 3 x 30 µg 30 µg ab273982 - GOLD-Hydrazide 1 x 4ml 1 x 4ml 1 x 1500µl ab273984 - GOLD-Hydrazide Antibody Diluent 1 x 500µl 1 x 500µl 1 x 500µl ab273985 - GOLD-Hydrazide Antibody Oxidation Reagent 1 x 90µg 3 x 30µg 1 x 30µg ab273983 - GOLD-Hydrazide Wash/Coupling Buffer 1 x 50ml 1 x 50ml 1 x 20ml -
Research areas
Images
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Fc Site Specific Labelling via Aldehyde Groups
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Lateral Flow AssayLateral flow assay demonstrates increased performance of site-specific (IgG-Gold Hydrazide) vs. random (IgG-Gold nanoparticle) labeling. All components of the lateral flow assay are identical with the exception of the method used to conjugate the oxidised IgG to Gold-Hydrazide or IgG to Gold nanoparticle. Oxidised IgG and IgG were derived from the same antibody.