Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181)
![Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181)](https://www.abcam.com/ps/products/272/ab272181/Images/ab272181-385440-goat-monoclonal-rmg06-antimouse-Igg-fc-biotin-elisa.jpg "Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181)")
Key features and details
- Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA
Overview
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Product name
Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181) reacts to the Fc region of Mouse IgG, including IgG1, IgG2a, and IgG2b. It shows very slight reaction with mouse IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.
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Tested applications
Suitable for: ELISAmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RMG06 -
Isotype
IgG -
Research areas
Images
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ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181)
ELISA of mouse immunoglobulins shows ab272181 reacts to the Fc region of mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG.
The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab272181 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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![ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181)](https://www.abcam.com/ps/products/272/ab272181/Images/ab272181-385439-biotin-antimouse-IgG-Fc-antibody-rmg06-titer-elisa-IgGs-different-species.JPG)
ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181)A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab272181 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.