Glycerophosphorylcholine Assay Kit (ab239717)
Key features and details
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Other biological fluids, Saliva, Tissue
Overview
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Product name
Glycerophosphorylcholine Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Saliva, Other biological fluids, Tissue -
Product overview
The Glycerophosphorylcholine Assay Kit (ab239717) allows a quick and sensitive means to determine GPC concentration in a biological sample. The assay is robust and specific, with a GPC enzyme mix converting GPC in the sample into an intermediate that is then utilized to quantitatively generate signal using the GPC Developer mix and GPC Probe.
The assay is rapid, simple, high throughput compatible, and can detect GPC concentrations as low as 0.1 μM in tissue lysates and other samples such as breast milk and seminal plasma.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests GPC Assay Buffer 1 x 25ml GPC Developer 1 vial GPC Enzyme Mix 1 vial GPC Probe (in DMSO) 1 x 200µl GPC Standard (1 µmole) 1 vial Sample Clean-Up Mix 1 vial -
Relevance
Glycerophosphorylcholine (GPC), also referred to as alpha-GPC or choline alfoscerate, is a small metabolite and potential dementia treatment. GPC is a breakdown product of phosphatidylcholine, and dysregulation of phospholipid metabolism can alter lipid and metabolite concentrations, leading to health problems such as fatty liver, Gaucher’s disease, and Tay-Sachs disease. GPC is also component of seminal fluid and breast milk, and a precursor of the neurotransmitter, acetylcholine. As GPC can efficiently cross the blood-brain barrier, it has been hypothesized as a treatment for Alzheimer’s disease and other forms of dementia. In such cases, GPC serves as a biomarker for lipid membrane decomposition.
Images
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Colorimetric GPC Standard Curve
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Fluorometric GPC Standard Curve. -
Determination of total GPC concentration in Liver lysate, Human Milk, and Seminal Fluid.For this experiment, 100 mg rat liver was homogenized and prepared according to the kit protocol. Breast Milk and Seminal Plasma were prepped according to the above protocol and treated with Sample Clean-Up Mix. Values were determined with fluorometric measurements and are mean ± standard deviation of at least three independent determinations.
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Specificity of the assay; PC = phosphatidylcholine, PA = phosphatidic acid, SM = sphingomyelin, GPC = glycerophosphorylcholine.