Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 1 mU/well
Overview
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Product name
Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
Species reactivity
Reacts with: Mammals, Other speciesProduct overview
Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to forms an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.
Visit our FAQs page for tips and troubleshooting.
Notes
Glycerol-3-Phosphate Dehydrogenase (EC 1.1.1.8) is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests GPDH Assay Buffer 1 x 27ml GPDH Positive Control 1 vial GPDH Probe 1 vial GPDH Substrate 1 vial NADH Standard 1 vial -
Research areas
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Cellular localization
Cytoplasmic -
Alternative names
- Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
- GPD-C
- GPDH-C
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests GPDH Assay Buffer 1 x 27ml GPDH Positive Control 1 vial GPDH Probe 1 vial GPDH Substrate 1 vial NADH Standard 1 vial -
Research areas
-
Cellular localization
Cytoplasmic -
Alternative names
- Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
- GPD-C
- GPDH-C
Images
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Glycerol-3-Phosphate Dehydrogenase (63PDH) Assay Kit (ab174095)
NADH measured in cell lysates showing quantity (nmol) per 1 mln cells after 30 min incubation time.
Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 2-18 fold.
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Glycerol-3-Phosphate Dehydrogenase (63PDH) Assay Kit (ab174095)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Measurement of Glycerol-3-Phosphate Dehydrogenase activityMeasurement of Glycerol-3-Phosphate Dehydrogenase activity in 3T3-L1 pre- adipocyte (60 µg) and differentiated 3T3-L1 adipocytes (60 µg) using Glycerol-3-Phosphate Dehydrogenase activity (GPDH) Assay Kit (Colorimetric) (ab174095). This is example data only.