Glutamate Dehydrogenase Activity Assay Kit (ab102527)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 mU/well
Overview
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Product name
Glutamate Dehydrogenase Activity Assay Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
> 0.01 mU/well -
Product overview
Glutamate Dehydrogenase Activity Assay Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
Visit our FAQs page for tips and troubleshooting. -
Notes
Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests GDH Assay Buffer WM 1 x 25ml GDH Developer Red 1 vial GDH Positive Control Green 1 vial Glutamate Blue 1 x 1ml NADH Yellow 1 vial -
Research areas
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Relevance
Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to alpha-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes. -
Alternative names
- GDH
Images
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Functional Studies - Glutamate dehydrogenase Detection Kit (ab102527)
GDH measured showing activity (mU) per mL of tested sample
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Functional Studies - Glutamate Dehydrogenase Detection Kit (ab102527GDH measured in cell lysates showing activity (mU) per 106 cells tested
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Functional Studies - Glutamate Dehydrogenase Detection Kit (ab102527GDH measured in various samples showing O.D.'s in time course
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Functional Studies - Glutamate Dehydrogenase Detection Kit (ab102527)NADH Standard Curve