Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners. Thus, detection of in vivo protein sumoylation (SUMO conjugation) would provide useful information for understanding SUMO modification as a regulatory control mechanisms for many nuclear proteins.

Global Protein Sumoylation Assay Kit (ab115131) allows the user to measure in vivo protein sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo protein sumoylation from multiple mammalian cells/tissues extracts.

• Epigenetics and Nuclear Signaling
• Chromatin Modifying Enzymes
• Sumoylation

• Kits/ Lysates/ Other
• Kits
• Epigenetic kits
• Sumoylation

• Epigenetics and Nuclear Signaling
• Assays & Kits
• Sumoylation