Formate Assay Kit (ab111748)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Overview
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Product name
Formate Assay Kit -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Quantitative -
Assay time
1h 00m -
Product overview
In Abcam's Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (? = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples
Visit our FAQs page for tips and troubleshooting. -
Notes
Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Formate Assay Buffer WM 1 x 25ml Formate Enzyme Mix - Lyophilized Green cap 1 vial Formate Standard (100 mM) Yellow cap 1 x 100µl Formate Substrate Mix- Lyophilized Red cap 1 vial -
Research areas
Images
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Functional Studies - Formate Assay Kit (ab111748)Example of standard curve obtained using ab111748