FFN511, Fluorescent substrate for VMAT2 (ab120331)
Key features and details
- First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity.
- CAS Number: 1004548-96-2
- Purity: > 98%
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
FFN511, Fluorescent substrate for VMAT2 -
Description
First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity. -
Alternative names
- Fluorescent False Neurotransmitter 511
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Biological description
First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity.
Inhibits 5-HT binding to VMAT2 (IC50 = 1 µM (comparable to dopamine itself)). Released via exocytosis in the striatum in mouse acute slice preparation. Labels dopamine and other presynaptic terminals in the striatum.
Compatible with GFP tags and other optical probes. Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy.
View the technique online
For highly selective labelling of dopaminergic presynaptic termainals FFN102 (ab120866) is recommended. -
Purity
> 98% -
CAS Number
1004548-96-2 -
Chemical structure
Properties
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Chemical name
9-(2-Aminoethyl)-2,3,6,7-tetrahydro-1H,5H,11H-[1]benzopyrano[6,7,8-ij]quinolizin-11-one -
Molecular weight
284.36 -
Molecular formula
C17H20N2O2 -
PubChem identifier
23725121 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
To make a 10 μM solution for slice incubation, 25 μl of a 20 mM FFN511 stock solution in DMSO is added to 50 ml of ACSF. This results in a 10 μM FFN511 solution in ACSF containing 0.05% DMSO.Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
NCCC2=CC(=O)Oc1c3CCCN4CCCc(cc12)c34 -
Source
Synthetic
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Research areas
