Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD63 / CD81) (ab267479)
Key features and details
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Plasma
Overview
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Product name
Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD63 / CD81)
See all Exosome kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Exosome 3 = 10% Inter-assay Sample n Mean SD CV% Exosomes 4 = 11% -
Sample type
Plasma -
Species reactivity
Reacts with: Human -
Product overview
Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD63/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
The kit is intended for the immunoisolation (immunomagnetic or FACS) and Flow Cytometry analysis of pre-enriched CD63+ human exosomes from plasma.
Visit our FAQs page for tips and troubleshooting.
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Notes
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 25 tests Anti-CD81 PE (Clone M38) 1 x 125µl Assay Buffer 10X 1 x 10ml CD63+ [Clone TEA3/18] capture beads 1 x 2500µl -
Relevance
Exosomes are best defined as extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. This liberates intraluminal vesicles (ILVs) into the extracellular milieu and the vesicles thereby released are what we know as exosomes. They contain abundant proteins, DNA, mRNA, and non-coding RNAs. They are now considered as an additional mechanism for intercellular communication, allowing cells to exchange proteins, lipids and genetic material. Increasing studies have shown that exosomes play an important role in tumour initiation, growth, progression, metastasis, drug resistance and immune escape.
Images
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Dot-plot gating strategy for acquisition and analysis
FSC vs SSC (A) and FL3 vs FL4 (B).
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Flow analysis of exosomes.Cell culture exosomes, pre-enriched using Total Exosome Isolation from PC3 Cell Culture Media (A) and human plasma (B), were resuspended in PBS and bound to CD63-capture beads during an overnight incubation. The following day the bead-bound exosomes were indirect stained with primary antibody detection (CD9-PE/CD81-PE).cytometry.
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Dynamic range of the assay analyzed by flow cytometryRelationship between background noise and specific signal at different exosome concentrations.
