Estrogen BPA Environmental ELISA Kit (ab175820)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 0.01 ng/ml - 1000 ng/ml
- Sample type: Plasma, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
Estrogen BPA Environmental ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall 9 0.08% Inter-assay Sample n Mean SD CV% overall 9 0.8% -
Sample type
Urine, Serum, Plasma, Tissue -
Assay type
Competitive -
Sensitivity
= 3 pg/ml -
Range
0.01 ng/ml - 1000 ng/ml -
Assay duration
Multiple steps standard assay -
Product overview
Abcam’s Estrogen BPA Environmental competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Estrogen BPA Environmental in urine, serum, plasma, cells and tissues following proper isolation and purification.
This competitive ELISA kit is based on competition between the Estrogen BPA Environmental epitope and the Estrogen BPA Environmental-HRP conjugate for a limited number of binding sites available from the anti- Estrogen BPA Environmental antibody; which is coated on the bottom of the wells of the 96 well ELISA plate. The conjugate concentration is held constant in each well, while the concentration of the Estrogen BPA Environmental is variable, based on the concentration of the sample or standard. Thus the amount of the Estrogen BPA Environmental conjugate which is able to bind to each of the wells is inversely proportional to the concentration of Estrogen BPA Environmental in the standard or sample. The amount of the conjugate which is bound to each well is then determined by the amount of color obtained, when TMB is added. The TMB reacts with the HRP available in the well. With the addition of sulfuric acid, the blue colored product is converted into a yellow colored product, which can be read on a plate reader at 450 nm.
Estrogen BPA Environmental is a phenolic environmental estrogen1 which disrupts endocrine activity. In human, a Estrogen BPA Environmental glucuronide was a primary metabolite of BPA. In a recent study, the age group with highest Estrogen BPA Environmental exposure was 6-11 years old with a mean total (free + glucuronidated) Estrogen BPA Environmental level of 4.33 ng/g of creatinine. Urinary Estrogen BPA Environmental levels were correlated with cardiovascular diseases and diabetes. A recent study revealed that a 12-ounce serving of canned soup for 5 days increased urinary Estrogen BPA Environmental level 12-fold due to Estrogen BPA Environmental -containing epoxy resin lining of the cans.
The free Estrogen BPA Environmental level in urine or cell culture media can be measured using the Estrogen BPA Environmental ELISA, without ethyl acetate extraction after 4-fold dilution of the sample. The glucuronidated Estrogen BPA Environmental level can also be measured without extraction after beta-glucuronidase treatment using our specific protocol.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests BPA Standard 1 x 2µl BPA-HRP Conjugates 1 x 12µl HRP Buffer 1 x 15ml Pre-coated 96-Well Microplate 1 unit 10X Sample Dilution Buffer 1 x 25ml TMB Substrate 1 x 24ml 10X Wash Buffer Solution 1 x 25ml -
Research areas
Images
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Typical Standard Curve
Example of standard curve obtained with ab175820.