ECL Western Blotting Substrate Kit (ab65623)
Overview
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Product name
ECL Western Blotting Substrate Kit -
Product overview
The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.
Our ECL kits include this kit ab65623 and our high sensitivity ECL substrate kits:
- High Sensitivity ECL Substrate Kit ab133406 to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per bandDetection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.
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Notes
Reagents provided are sufficient for 400 cm2 of membrane.
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Tested applications
Suitable for: WBmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 50 tests 500 tests Reagent A 1 x 25ml 1 x 250ml Reagent B 1 x 25ml 1 x 250ml -
Research areas
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Relevance
ECL (enhanced luminol-based chemiluminescent) detection is a sensitive, nonradioactive method for easy detection of horseradish peroxidase (HRP) on western blots. -
Alternative names
- Enhanced luminol based chemiluminescent
Images
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ECL Western Blotting Substrate Kit - 50 Tests (ab65623)ECL Western Blotting Substrate
